class i hdacs Search Results


hdac class i/ii substrate  (Promega)
90
Promega hdac class i/ii substrate
Effect of Rhein on <t>HDAC</t> activity and G2/M cell cycle phase regulation, p53 and p21 abundance and proliferation markers. ( A ) Representative blots and quantification showing Rhein-mediated increase of p53 and p21 abundance (n = 4). ( B ) Representative blots and quantification showing Rhein-driven p53 stabilization by increased HDAC inhibition-mediated acetylation at Lys382 (n = 4). 1 mM sodium butyrate (SB) was used as reference HDAC inhibitor. ( C ) Representative blots and quantification showing Rhein-mediated increase of p53 abundance (n = 4) under conditions described in ( B ). ( D ) Representative blots and quantification showing Rhein-mediated increase of p21 abundance (n = 4) under conditions described in ( B ). Antibodies in ( C , D ) were probed to the same membrane. ( E ) Enzymatic HDAC activity is inhibited in the presence of Rhein. Graph showing HDAC activity after 30 min treatment of normal cell lysates with Rhein or SB (n = 4). All data are presented as mean ± SD. One-way-ANOVA with post-hoc Sidak’s multiple comparison, *p < 0.05, **p < 0.01, ***p < 0.001, as indicated.
Hdac Class I/Ii Substrate, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/class+i+hdacs/pmc07078222-216-18-28?v=Promega
Average 90 stars, based on 1 article reviews
hdac class i/ii substrate - by Bioz Stars, 2026-07
90/100 stars
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fluorogenic class i hdac substrate  (Bachem)
90
Bachem fluorogenic class i hdac substrate
Effect of Rhein on <t>HDAC</t> activity and G2/M cell cycle phase regulation, p53 and p21 abundance and proliferation markers. ( A ) Representative blots and quantification showing Rhein-mediated increase of p53 and p21 abundance (n = 4). ( B ) Representative blots and quantification showing Rhein-driven p53 stabilization by increased HDAC inhibition-mediated acetylation at Lys382 (n = 4). 1 mM sodium butyrate (SB) was used as reference HDAC inhibitor. ( C ) Representative blots and quantification showing Rhein-mediated increase of p53 abundance (n = 4) under conditions described in ( B ). ( D ) Representative blots and quantification showing Rhein-mediated increase of p21 abundance (n = 4) under conditions described in ( B ). Antibodies in ( C , D ) were probed to the same membrane. ( E ) Enzymatic HDAC activity is inhibited in the presence of Rhein. Graph showing HDAC activity after 30 min treatment of normal cell lysates with Rhein or SB (n = 4). All data are presented as mean ± SD. One-way-ANOVA with post-hoc Sidak’s multiple comparison, *p < 0.05, **p < 0.01, ***p < 0.001, as indicated.
Fluorogenic Class I Hdac Substrate, supplied by Bachem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/class+i+hdacs/10__12688_slash_f1000research__2___238__v2-85-10-20?v=Bachem
Average 90 stars, based on 1 article reviews
fluorogenic class i hdac substrate - by Bioz Stars, 2026-07
90/100 stars
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hdac class i inhibitor az03  (AstraZeneca ltd)
90
AstraZeneca ltd hdac class i inhibitor az03
Effect of Rhein on <t>HDAC</t> activity and G2/M cell cycle phase regulation, p53 and p21 abundance and proliferation markers. ( A ) Representative blots and quantification showing Rhein-mediated increase of p53 and p21 abundance (n = 4). ( B ) Representative blots and quantification showing Rhein-driven p53 stabilization by increased HDAC inhibition-mediated acetylation at Lys382 (n = 4). 1 mM sodium butyrate (SB) was used as reference HDAC inhibitor. ( C ) Representative blots and quantification showing Rhein-mediated increase of p53 abundance (n = 4) under conditions described in ( B ). ( D ) Representative blots and quantification showing Rhein-mediated increase of p21 abundance (n = 4) under conditions described in ( B ). Antibodies in ( C , D ) were probed to the same membrane. ( E ) Enzymatic HDAC activity is inhibited in the presence of Rhein. Graph showing HDAC activity after 30 min treatment of normal cell lysates with Rhein or SB (n = 4). All data are presented as mean ± SD. One-way-ANOVA with post-hoc Sidak’s multiple comparison, *p < 0.05, **p < 0.01, ***p < 0.001, as indicated.
Hdac Class I Inhibitor Az03, supplied by AstraZeneca ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/class+i+hdacs/pm35433850-66-138-117?v=AstraZeneca+ltd
Average 90 stars, based on 1 article reviews
hdac class i inhibitor az03 - by Bioz Stars, 2026-07
90/100 stars
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class i hdac inhibitors  (Huntsman International LLC)
90
Huntsman International LLC class i hdac inhibitors
Effect of Rhein on <t>HDAC</t> activity and G2/M cell cycle phase regulation, p53 and p21 abundance and proliferation markers. ( A ) Representative blots and quantification showing Rhein-mediated increase of p53 and p21 abundance (n = 4). ( B ) Representative blots and quantification showing Rhein-driven p53 stabilization by increased HDAC inhibition-mediated acetylation at Lys382 (n = 4). 1 mM sodium butyrate (SB) was used as reference HDAC inhibitor. ( C ) Representative blots and quantification showing Rhein-mediated increase of p53 abundance (n = 4) under conditions described in ( B ). ( D ) Representative blots and quantification showing Rhein-mediated increase of p21 abundance (n = 4) under conditions described in ( B ). Antibodies in ( C , D ) were probed to the same membrane. ( E ) Enzymatic HDAC activity is inhibited in the presence of Rhein. Graph showing HDAC activity after 30 min treatment of normal cell lysates with Rhein or SB (n = 4). All data are presented as mean ± SD. One-way-ANOVA with post-hoc Sidak’s multiple comparison, *p < 0.05, **p < 0.01, ***p < 0.001, as indicated.
Class I Hdac Inhibitors, supplied by Huntsman International LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/class+i+hdacs/pmc06893361__EMBR___20___e48375___s012-3-0-61?v=Huntsman+International+LLC
Average 90 stars, based on 1 article reviews
class i hdac inhibitors - by Bioz Stars, 2026-07
90/100 stars
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class i hdacs  (MIDAC Corporation)
90
MIDAC Corporation class i hdacs
Effect of Rhein on <t>HDAC</t> activity and G2/M cell cycle phase regulation, p53 and p21 abundance and proliferation markers. ( A ) Representative blots and quantification showing Rhein-mediated increase of p53 and p21 abundance (n = 4). ( B ) Representative blots and quantification showing Rhein-driven p53 stabilization by increased HDAC inhibition-mediated acetylation at Lys382 (n = 4). 1 mM sodium butyrate (SB) was used as reference HDAC inhibitor. ( C ) Representative blots and quantification showing Rhein-mediated increase of p53 abundance (n = 4) under conditions described in ( B ). ( D ) Representative blots and quantification showing Rhein-mediated increase of p21 abundance (n = 4) under conditions described in ( B ). Antibodies in ( C , D ) were probed to the same membrane. ( E ) Enzymatic HDAC activity is inhibited in the presence of Rhein. Graph showing HDAC activity after 30 min treatment of normal cell lysates with Rhein or SB (n = 4). All data are presented as mean ± SD. One-way-ANOVA with post-hoc Sidak’s multiple comparison, *p < 0.05, **p < 0.01, ***p < 0.001, as indicated.
Class I Hdacs, supplied by MIDAC Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/class+i+hdacs/pmc07043024-169-11-17?v=MIDAC+Corporation
Average 90 stars, based on 1 article reviews
class i hdacs - by Bioz Stars, 2026-07
90/100 stars
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brain class i hdac inhibitor (rbc1hi)  (Regenacy Pharmaceuticals)
90
Regenacy Pharmaceuticals brain class i hdac inhibitor (rbc1hi)
Effect of Rhein on <t>HDAC</t> activity and G2/M cell cycle phase regulation, p53 and p21 abundance and proliferation markers. ( A ) Representative blots and quantification showing Rhein-mediated increase of p53 and p21 abundance (n = 4). ( B ) Representative blots and quantification showing Rhein-driven p53 stabilization by increased HDAC inhibition-mediated acetylation at Lys382 (n = 4). 1 mM sodium butyrate (SB) was used as reference HDAC inhibitor. ( C ) Representative blots and quantification showing Rhein-mediated increase of p53 abundance (n = 4) under conditions described in ( B ). ( D ) Representative blots and quantification showing Rhein-mediated increase of p21 abundance (n = 4) under conditions described in ( B ). Antibodies in ( C , D ) were probed to the same membrane. ( E ) Enzymatic HDAC activity is inhibited in the presence of Rhein. Graph showing HDAC activity after 30 min treatment of normal cell lysates with Rhein or SB (n = 4). All data are presented as mean ± SD. One-way-ANOVA with post-hoc Sidak’s multiple comparison, *p < 0.05, **p < 0.01, ***p < 0.001, as indicated.
Brain Class I Hdac Inhibitor (Rbc1hi), supplied by Regenacy Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/class+i+hdacs/pm37291337-62-14-16?v=Regenacy+Pharmaceuticals
Average 90 stars, based on 1 article reviews
brain class i hdac inhibitor (rbc1hi) - by Bioz Stars, 2026-07
90/100 stars
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selective class i hdac inhibitor entinostat ms275  (ChemScene llc)
90
ChemScene llc selective class i hdac inhibitor entinostat ms275
Effect of Rhein on <t>HDAC</t> activity and G2/M cell cycle phase regulation, p53 and p21 abundance and proliferation markers. ( A ) Representative blots and quantification showing Rhein-mediated increase of p53 and p21 abundance (n = 4). ( B ) Representative blots and quantification showing Rhein-driven p53 stabilization by increased HDAC inhibition-mediated acetylation at Lys382 (n = 4). 1 mM sodium butyrate (SB) was used as reference HDAC inhibitor. ( C ) Representative blots and quantification showing Rhein-mediated increase of p53 abundance (n = 4) under conditions described in ( B ). ( D ) Representative blots and quantification showing Rhein-mediated increase of p21 abundance (n = 4) under conditions described in ( B ). Antibodies in ( C , D ) were probed to the same membrane. ( E ) Enzymatic HDAC activity is inhibited in the presence of Rhein. Graph showing HDAC activity after 30 min treatment of normal cell lysates with Rhein or SB (n = 4). All data are presented as mean ± SD. One-way-ANOVA with post-hoc Sidak’s multiple comparison, *p < 0.05, **p < 0.01, ***p < 0.001, as indicated.
Selective Class I Hdac Inhibitor Entinostat Ms275, supplied by ChemScene llc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/class+i+hdacs/pm31070057-40-6-8?v=ChemScene+llc
Average 90 stars, based on 1 article reviews
selective class i hdac inhibitor entinostat ms275 - by Bioz Stars, 2026-07
90/100 stars
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hdac class i  (TranScrip Partners)
90
TranScrip Partners hdac class i
Effect of Rhein on <t>HDAC</t> activity and G2/M cell cycle phase regulation, p53 and p21 abundance and proliferation markers. ( A ) Representative blots and quantification showing Rhein-mediated increase of p53 and p21 abundance (n = 4). ( B ) Representative blots and quantification showing Rhein-driven p53 stabilization by increased HDAC inhibition-mediated acetylation at Lys382 (n = 4). 1 mM sodium butyrate (SB) was used as reference HDAC inhibitor. ( C ) Representative blots and quantification showing Rhein-mediated increase of p53 abundance (n = 4) under conditions described in ( B ). ( D ) Representative blots and quantification showing Rhein-mediated increase of p21 abundance (n = 4) under conditions described in ( B ). Antibodies in ( C , D ) were probed to the same membrane. ( E ) Enzymatic HDAC activity is inhibited in the presence of Rhein. Graph showing HDAC activity after 30 min treatment of normal cell lysates with Rhein or SB (n = 4). All data are presented as mean ± SD. One-way-ANOVA with post-hoc Sidak’s multiple comparison, *p < 0.05, **p < 0.01, ***p < 0.001, as indicated.
Hdac Class I, supplied by TranScrip Partners, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/class+i+hdacs/10__1097_slash_ta__0b013e31818233ef-188-7-78?v=TranScrip+Partners
Average 90 stars, based on 1 article reviews
hdac class i - by Bioz Stars, 2026-07
90/100 stars
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class i and ii hdac-containing hela cell extract  (Biomol GmbH)
90
Biomol GmbH class i and ii hdac-containing hela cell extract
Effect of Rhein on <t>HDAC</t> activity and G2/M cell cycle phase regulation, p53 and p21 abundance and proliferation markers. ( A ) Representative blots and quantification showing Rhein-mediated increase of p53 and p21 abundance (n = 4). ( B ) Representative blots and quantification showing Rhein-driven p53 stabilization by increased HDAC inhibition-mediated acetylation at Lys382 (n = 4). 1 mM sodium butyrate (SB) was used as reference HDAC inhibitor. ( C ) Representative blots and quantification showing Rhein-mediated increase of p53 abundance (n = 4) under conditions described in ( B ). ( D ) Representative blots and quantification showing Rhein-mediated increase of p21 abundance (n = 4) under conditions described in ( B ). Antibodies in ( C , D ) were probed to the same membrane. ( E ) Enzymatic HDAC activity is inhibited in the presence of Rhein. Graph showing HDAC activity after 30 min treatment of normal cell lysates with Rhein or SB (n = 4). All data are presented as mean ± SD. One-way-ANOVA with post-hoc Sidak’s multiple comparison, *p < 0.05, **p < 0.01, ***p < 0.001, as indicated.
Class I And Ii Hdac Containing Hela Cell Extract, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/class+i+hdacs/pm16335928-237-21-24?v=Biomol+GmbH
Average 90 stars, based on 1 article reviews
class i and ii hdac-containing hela cell extract - by Bioz Stars, 2026-07
90/100 stars
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class i hdac antibody sampler kit  (Cell Signaling Technology Inc)
N/A
Acetylation of the histone tail causes chromatin to adopt an ",open", conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights
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Image Search Results


Effect of Rhein on HDAC activity and G2/M cell cycle phase regulation, p53 and p21 abundance and proliferation markers. ( A ) Representative blots and quantification showing Rhein-mediated increase of p53 and p21 abundance (n = 4). ( B ) Representative blots and quantification showing Rhein-driven p53 stabilization by increased HDAC inhibition-mediated acetylation at Lys382 (n = 4). 1 mM sodium butyrate (SB) was used as reference HDAC inhibitor. ( C ) Representative blots and quantification showing Rhein-mediated increase of p53 abundance (n = 4) under conditions described in ( B ). ( D ) Representative blots and quantification showing Rhein-mediated increase of p21 abundance (n = 4) under conditions described in ( B ). Antibodies in ( C , D ) were probed to the same membrane. ( E ) Enzymatic HDAC activity is inhibited in the presence of Rhein. Graph showing HDAC activity after 30 min treatment of normal cell lysates with Rhein or SB (n = 4). All data are presented as mean ± SD. One-way-ANOVA with post-hoc Sidak’s multiple comparison, *p < 0.05, **p < 0.01, ***p < 0.001, as indicated.

Journal: Scientific Reports

Article Title: Rhein, a novel Histone Deacetylase (HDAC) inhibitor with antifibrotic potency in human myocardial fibrosis

doi: 10.1038/s41598-020-61886-3

Figure Lengend Snippet: Effect of Rhein on HDAC activity and G2/M cell cycle phase regulation, p53 and p21 abundance and proliferation markers. ( A ) Representative blots and quantification showing Rhein-mediated increase of p53 and p21 abundance (n = 4). ( B ) Representative blots and quantification showing Rhein-driven p53 stabilization by increased HDAC inhibition-mediated acetylation at Lys382 (n = 4). 1 mM sodium butyrate (SB) was used as reference HDAC inhibitor. ( C ) Representative blots and quantification showing Rhein-mediated increase of p53 abundance (n = 4) under conditions described in ( B ). ( D ) Representative blots and quantification showing Rhein-mediated increase of p21 abundance (n = 4) under conditions described in ( B ). Antibodies in ( C , D ) were probed to the same membrane. ( E ) Enzymatic HDAC activity is inhibited in the presence of Rhein. Graph showing HDAC activity after 30 min treatment of normal cell lysates with Rhein or SB (n = 4). All data are presented as mean ± SD. One-way-ANOVA with post-hoc Sidak’s multiple comparison, *p < 0.05, **p < 0.01, ***p < 0.001, as indicated.

Article Snippet: Cell lysates were prepared using 1% NP‐40 (in 1x PBS) and 5 μg of protein was incubated with HDAC class I/II substrate (HDAC-Glo I/II Assay and Screening System, Promega, Madison, IA, USA) for 45 min (RT), before luminescence was determined.

Techniques: Activity Assay, Inhibition, Membrane, Comparison

Rhein functionally inhibits TGFβ1-stimulated FMT. ( A ) Representative Western Blot and quantification of αSMA relative protein abundance (n = 4). ( B ) Rhein inhibition of TGFβ1/SMAD signaling. Representative blot and quantification of phospho(Ser465/467)-SMAD2 abundance in Rhein and TGFβ1 treated cells. ( C ) Expression analysis of SMAD7 showing TGFβ1-mediated increase of transcription and absence of Rhein-mediated effects (n = 4). ( D ) Rhein increases SMAD7 abundance independently from TGFβ1. Representative blot and quantification showing increased basal SMAD7 abundance after Rhein treatment and TGFβ1-dependent SMAD7 expression (n = 4). ( E ) Effect of HDAC inhibition on SMAD7 stabilization. Representative blot and quantification showing increased protein abundance after Rhein and SB treatment (N = 4). ( F ) FPCL contraction assay. Quantitative analysis on the contraction of experimental FPCLs (n = 4). Representative overhead pictures of FPCLs before (d0) and after treatment (d0) with TGFβ1 alone or in combination with Rhein. Dashed line indicates baseline (start point at d0). All data are presented as mean ± SD. One-way-ANOVA with post-hoc Sidak’s multiple comparison, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 as indicated.

Journal: Scientific Reports

Article Title: Rhein, a novel Histone Deacetylase (HDAC) inhibitor with antifibrotic potency in human myocardial fibrosis

doi: 10.1038/s41598-020-61886-3

Figure Lengend Snippet: Rhein functionally inhibits TGFβ1-stimulated FMT. ( A ) Representative Western Blot and quantification of αSMA relative protein abundance (n = 4). ( B ) Rhein inhibition of TGFβ1/SMAD signaling. Representative blot and quantification of phospho(Ser465/467)-SMAD2 abundance in Rhein and TGFβ1 treated cells. ( C ) Expression analysis of SMAD7 showing TGFβ1-mediated increase of transcription and absence of Rhein-mediated effects (n = 4). ( D ) Rhein increases SMAD7 abundance independently from TGFβ1. Representative blot and quantification showing increased basal SMAD7 abundance after Rhein treatment and TGFβ1-dependent SMAD7 expression (n = 4). ( E ) Effect of HDAC inhibition on SMAD7 stabilization. Representative blot and quantification showing increased protein abundance after Rhein and SB treatment (N = 4). ( F ) FPCL contraction assay. Quantitative analysis on the contraction of experimental FPCLs (n = 4). Representative overhead pictures of FPCLs before (d0) and after treatment (d0) with TGFβ1 alone or in combination with Rhein. Dashed line indicates baseline (start point at d0). All data are presented as mean ± SD. One-way-ANOVA with post-hoc Sidak’s multiple comparison, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 as indicated.

Article Snippet: Cell lysates were prepared using 1% NP‐40 (in 1x PBS) and 5 μg of protein was incubated with HDAC class I/II substrate (HDAC-Glo I/II Assay and Screening System, Promega, Madison, IA, USA) for 45 min (RT), before luminescence was determined.

Techniques: Western Blot, Quantitative Proteomics, Inhibition, Expressing, Contraction Assay, Comparison